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KMID : 0525720140190040292
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Journal of Chitin and Chitosan
2014 Volume.19 No. 4 p.292 ~ p.296
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Biotransformation of Ginsenoside Rb1 to Ginsenoside Rd by β-glucosidase from Lactobacillus plantarum G1
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Kim Yeong-Su
Kim Do-Yeon
Shon Mi-Yae
Park Chang-Su
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Abstract
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The β-glucosidase from Lactobacillus plantarum G1, isolated from Kimchi, was purified as a single 50 kDa band by ammonium sulfate precipitation, HiTrap Q Hp, and Resource Q column chromatography, with a specific activity of 37 U/mg. The β- glucosidase L. plantarum G1 showed optimum activity at pH 4.0 and 40℃ for ginsenoside Rd production. The β-glucosidase from L. plantarum G1 hydrolyze only outer β-(1,2)-D-glucose linkage at C-20 position in ginsenosides which has narrow substrate specificity for ginsenoside. Under the optimum conditins, the enzyme produced 1 mM of ginsenoside Rd from 1 mM of ginsenoside Rb1 after 120 min, with molar yield of 100%.
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KEYWORD
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Ginsenoside Rd, Lactobacillus plantarum, Minor ginsenoside, Enzyme purification
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